小鼠抗SETD7單克隆抗體

Background:

Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.

Applications:

WB, IHC, IF, FC

Name of antibody:

SETD7

Immunogen:

Fusion protein of human SETD7

Full name:

SET domain containing (lysine methyltransferase) 7 (SETD7)

Synonyms:

KMT7; SET7; SET7/9; SET9

SwissProt:

Q8WTS6

IHC positive control:

adenocarcinoma of human endometrium tissue and carcinoma of human bladder tissue

IHC Recommend dilution:

30-150

WB Predicted band size:

41 kDa

WB Positive control:

Hela, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF-7 cell lysates

WB Recommended dilution:

500-2000