中文名稱: RAW264.7細(xì)胞裂解液
英文名稱: RAW264.7 Cell Lysate
別 名: RAW264.7細(xì)胞裂解液
相關(guān)類別: 抗原
儲(chǔ) 存: 冷凍(-20℃) 避光
概述
RAW264.7 cell lysates from Mouse peritoneal macrophages cells were cultured in the media containing 10% FBS. |
技術(shù)規(guī)格
Background
Cells were lysed in lysis buffer (50mM Tris(PH7.4), 150mM NaCl, 1% NP-40, 1% SDS). 100 μg of cellular protein was mixed with 4 times volume of 5X SDS-PAGE loading buffer.