小鼠抗ACLY(C-term)單克隆抗體

Background:

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.

Applications:

WB, IF, FC

Name of antibody:

ACLY(C-term)

Immunogen:

Fusion protein of human ACLY

Full name:

ATP citrate lyase

Synonyms:

ACL; ATPCL; CLATP

SwissProt:

P53396

WB Predicted band size:

121 kDa

WB Positive control:

Hela, K562, COS7 and 3T3 cell lysates

WB Recommended dilution:

1000-5000

IF positive control:

Hela cells

IF Recommend dilution:

50-150

FC Recommend dilution:

50-100