小鼠抗PSMD4單克隆抗體

Background:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21.

Applications:

ELISA, WB

Name of antibody:

PSMD4

Immunogen:

Fusion protein of human PSMD4

Full name:

proteasome 26S subunit, non-ATPase 4

Synonyms:

AF; ASF; S5A; AF-1; MCB1; Rpn10; pUB-R5

SwissProt:

P55036

WB Predicted band size:

41 kDa

WB Positive control:

Jurkat whole cell lysate

WB Recommended dilution:

500-2000