購物車
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021-54845833/15800441009
中文名稱： 兔抗NEDD8多克隆抗體
英文名稱： Anti-NEDD8 rabbit polyclonal antibody
別 名： NEDD8; FLJ43224; MGC104393; MGC125896; MGC125897; Nedd-8
相關(guān)類別： 一抗
儲 存： 冷凍（-20℃） 避光
宿 主： Rabbit
抗 原： NEDD8
反應(yīng)種屬： Human, Mouse, Rat
標 記 物： Unconjugate
克隆類型： rabbit polyclonal
技術(shù)規(guī)格
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Background: |
Neural precursor cell-expressed developmentally downregulated protein 8 (NEDD8), also known as Rub1 (related to ubiquitin 1) in plants and yeast, is a member of the ubiquitin-like protein family. The covalent attachment of NEDD8 to target proteins, termed neddylation, is a reversible, multi-step process analogous to ubiquitination. NEDD8 is first synthesized in a precursor form with a carboxy-terminal extension peptide that is removed by either the UCH-L3 or NEDP1/DEN1 hydrolase protein to yield a mature NEDD8 protein. Mature NEDD8 is then covalently linked to target proteins via the carboxy-terminal glycine residue in a reaction catalyzed by the APP-BP1/Uba3 heterodimer complex and Ubc12 as the E1- and E2-like enzymes, respectively. An E3 ligase protein, Roc1/Rbx1, is also required for neddylation of the cullin proteins. Protein de-neddylation is catalyzed by a number of enzymes in the cell, including a "ubiquitin-specific" protease USP21, the NEDP1/DEN1 hydrolase and the COP9/signalosome (CSN). In contrast to the ubiquitin pathway, the NEDD8 modification system acts on only a few substrates and does not appear to target proteins for degradation. Neddylation of cullin proteins activates the SCF (Skp1-Cullin-F-box) E3 ubiquitin ligase complex by promoting complex formation and enhancing the recruitment of the E2-ubiquitin intermediate. While NEDD8 modification of VHL is not required for ubiquitination of HIF1-α, it is required for fibronectin matrix assembly. Mdm2-dependent neddylation of p53 inhibits its transcriptional activity. |
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Applications: |
WB |
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Name of antibody: |
NEDD8 |
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Immunogen: |
Fusion protein of human NEDD8 |
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Full name: |
neural precursor cell expressed, developmentally down-regulated 8 |
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Synonyms： |
NEDD8; FLJ43224; MGC104393; MGC125896; MGC125897; Nedd-8 |
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SwissProt: |
Q15843 |
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WB Predicted band size: |
9 kDa |
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WB Positive control: |
COS1 and Jurkat cells, brain tissue |
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WB Recommended dilution: |
500-2000 |
