兔抗SHC1 (Phospho-Tyr427)多克隆抗體

Background:

Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span

Applications:

WB, IHC, IF

Name of antibody:

SHC1 (Phospho-Tyr427)

Immunogen:

Synthetic peptide of human SHC1 (Phospho-Tyr427)

Full name:

SHC (Src homology 2 domain containing) transforming protein 1 (Phospho-Tyr427)

Synonyms:

SHC; SHCA

SwissProt:

P29353

IHC positive control:

Human breast carcinoma

IHC Recommend dilution:

50-100

WB Predicted band size:

63 kDa

WB Positive control:

293 cells untreated or treated with PMA

WB Recommended dilution:

500-1000

Positive control:

Hela cells

Recommended dilution:

100-200